Products

Biotinylated Human HLA-A*02:01&B2M&MAGE-A3 Monomer

Overview Specification

Product Name	Biotinylated Human HLA-A*02:01&B2M&MAGE-A3 Monomer
Cat. No.	MHCRP-0360
Category	Proteins & Peptides
Product Description	Recombinant Biotinylated Human HLA-A02:01&B2M&MAGE-A3 (KVAELVHFL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A02:01), Ile21-Met119 (B2M) and KVAELVHFL peptide.
Size	100/500 μg
Format	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped at ambient temperature.
Lead Time	Product delivery time depends on the supplier.
Species	Human
Expression Host	HEK293
Accession No	A0A140T913(HLA-A*02:01)&P61769(B2M)&KVAELVHFL
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Type	Monomer
Molecular Weight (MV)	The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Gene Synonyms	MAGE-A3
Endotoxin Residue	Less than 1 EU per μg by the LAL method.
Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC.
Amino Acid Range	Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M), and KVAELVHFL peptide
Product Background	Melanoma antigen family A, 3 (MAGE-A3) is a cancer-testis antigen whose expression has been demonstrated in a wide array of malignancies including melanoma, brain, breast, lung and ovarian cancer. In addition, its ability to elicit spontaneous humoral and cellular immune responses has been shown in cancer patients.
Allele	HLA-A*02:01
Biotinylated	Yes

For research or industrial raw materials, not for personal medical use!