Products

Biotinylated Human HLA-A*02:01&B2M&IGKC Monomer

Overview Specification

Product Name	Biotinylated Human HLA-A*02:01&B2M&IGKC Monomer
Cat. No.	MHCRP-0351
Category	Proteins & Peptides
Product Description	Recombinant Biotinylated Human HLA-A02:01&B2M&IGKC (FIFPPSDEQL) Monomer Protein is expressed from E. coli* with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and FIFPPSDEQL peptide.
Size	100/500 μg
Format	Liquid. Supplied as 0.22 μm filtered solution in 20 mM Tris, 200 mM NaCl (pH 8.0).
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped at ambient temperature.
Lead Time	Product delivery time depends on the supplier.
Species	Human
Expression Host	E. coli
Accession No	A0A140T913(HLA-A*02:01)&P61769(B2M)&FIFPPSDEQL
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Type	Monomer
Molecular Weight (MV)	The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Gene Synonyms	FIFPPSDEQL
Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC.
Amino Acid Range	Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M), and FIFPPSDEQL peptide
Activity	Immobilized biotinylated human HLA-A*02:01&B2M&IGKC (FIFPPSDEQL) monomer, His tag at 1 μg/mL (100 μL/well) on the streptavidin precoated plate (5 μg/mL). Dose response curve for anti-B2M antibody, mFc tag with the EC50 of 27.4 ng/mL determined by ELISA.
Product Background	IGKC (Immunoglobulin kappa constant) is a segment of the kappa light chain. This region is important for the stability and function of the antibody molecule, as it helps in the proper folding and assembly of the immunoglobulin. Additionally, the kappa constant region may also be involved in the interaction between the antibody and other immune system components.
Allele	HLA-A*02:01
Biotinylated	Yes

For research or industrial raw materials, not for personal medical use!