Products

Biotinylated Human HLA-A*02:01&B2M&CMV pp65 Monomer, E. coli

Overview Specification

Product Name	Biotinylated Human HLA-A*02:01&B2M&CMV pp65 Monomer, E. coli
Cat. No.	MHCRP-0342
Category	Proteins & Peptides
Product Description	Recombinant Biotinylated Human HLA-A02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein is expressed from E. coli* with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and NLVPMVATV peptide.
Size	100/500 μg
Format	Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 200 mM NaCl (pH 8.0). Normally 8% mannitol is added as protectant before lyophilization.
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped with dry ice.
Lead Time	Product delivery time depends on the supplier.
Species	Human
Expression Host	E. coli
Accession No	A0A140T913(HLA-A*02:01)&P61769(B2M)&NLVPMVATV
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Type	Monomer
Molecular Weight (MV)	The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Gene Synonyms	CMVpp65; NLVPMVATV
Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC.
Amino Acid Range	Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M), and NLVPMVATV peptide
Activity	Immobilized biotinylated HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) monomer, His tag at 1 μg/mL (100 μL/well) on the streptavidin precoated plate (5 μg/mL). Dose response curve for anti-B2M antibody, mFc tag with the EC50 of 12.1 ng/mL determined by ELISA.
Product Background	Human cytomegalovirus (CMV), a β-herpes virus with a double-stranded DNA, infects a wide variety of cells and establishes latency in the host. CMVpp65, a tegument protein of the herpes virus CMV, is the main viral antigen found in peripheral blood mononuclear cells (PBMCs) after viral infection and may activate cell-mediated immunity, accounting for 70-90% of the cytotoxic CD8+ T cells' (CTLs) response to CMV. Among the pp65-derived CTL epitope peptides, the 9-mer peptide 495NLVPMVATV503 (CMVpp65 495-503 peptide) is the most immunogenic T cell epitope predominantly displayed on HLA-A*02:01, the most common MHC-I allele in the population.
Allele	HLA-A*02:01
Biotinylated	Yes

For research or industrial raw materials, not for personal medical use!