Products

Biotinylated Human HLA-A*02:01&B2M&ARF Monomer

Overview Specification

Product Name	Biotinylated Human HLA-A*02:01&B2M&ARF Monomer
Cat. No.	MHCRP-0340
Category	Proteins & Peptides
Product Description	Recombinant Biotinylated Human HLA-A02:01&B2M&ARF (AAPGAPAAV) Monomer Protein is expressed from E. coli* with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and AAPGAPAAV peptide.
Size	100/500 μg
Format	Liquid. Supplied as 0.22 μm filtered solution in 20 mM Tris, 200 mM NaCl (pH 8.0).
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped at ambient temperature.
Lead Time	Product delivery time depends on the supplier.
Species	Human
Expression Host	E. coli
Accession No	A0A140T913(HLA-A*02:01)&P61769(B2M)&AAPGAPAAV
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Type	Monomer
Molecular Weight (MV)	The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Gene Synonyms	AAPGAPAAV; ARF
Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC.
Amino Acid Range	Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M), and AAPGAPAAV peptide
Activity	Immobilized biotinylated human HLA-A*02:01&B2M&ARF (AAPGAPAAV) monomer, His tag at 1 μg/mL (100 μL/well) on the streptavidin precoated plate (5 μg/mL). Dose response curve for anti-B2M antibody, mFc tag with the EC50 of 8.3 ng/mL determined by ELISA.
Product Background	Auxin Response Factors (ARFs) are a family of plant-specific transcription factors that play crucial roles in regulating plant growth and development. ARFs bind to auxin response elements (AuxREs) in the promoters of auxin-responsive genes, where they activate or repress transcription. They also recruit a second family of transcription factors, the Aux/IAA repressors, which confer an auxin response to the genes.
Allele	HLA-A*02:01
Biotinylated	Yes

For research or industrial raw materials, not for personal medical use!