Products

Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 Monomer

Overview Specification

Product Name	Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 Monomer
Cat. No.	MHCRP-0337
Category	Proteins & Peptides
Product Description	Recombinant Biotinylated Human HLA-A01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer Protein is expressed from HEK293 with His tag and Avi Tag at the C-terminus. It contains Gly25-Thr305 (HLA-A01:01), Ile21-Met119 (B2M) and EVDPIGHLY peptide.
Size	100/500 μg
Format	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped at ambient temperature.
Lead Time	Product delivery time depends on the supplier.
Species	Human
Expression Host	HEK293
Accession No	Q5SUL5(HLA-A*01:01)&P61769(B2M)&EVDPIGHLY
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Type	Monomer
Molecular Weight (MV)	The protein has a predicted MW of 50.30 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.
Gene Synonyms	Melanoma-associated antigen 3; MZ2-D; MZ2D; MZ2-D; CT1.3; MAGE-3
Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC.
Amino Acid Range	Gly25-Thr305 (HLA-A*01:01), Ile21-Met119 (B2M), and EVDPIGHLY peptide
Activity	Immobilized biotinylated human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) monomer, His tag at 1 μg/mL (100 μL/well) on the streptavidin precoated plate (5 μg/mL). Dose response curve for anti-B2M antibody, mFc tag with the EC50 of 10.9 ng/mL determined by ELISA (QC Test).
Product Background	Melanoma antigen gene A3 (MAGE-A3) is one of the most immunogenic cancer testis antigens and is common in various types of cancers. MAGE-A3 can be considered as a predictor for poor prognosis and an option for vaccine immunotherapy in patients with PCA.
Allele	HLA-A*01:01
Biotinylated	Yes

For research or industrial raw materials, not for personal medical use!