Products

APC-Labeled Human HLA-A*02:01&B2M&P53 R175H Tetramer

Overview Specification

Product Name	APC-Labeled Human HLA-A*02:01&B2M&P53 R175H Tetramer
Cat. No.	MHCRP-0330
Category	Proteins & Peptides
Product Description	Recombinant APC-Labeled Human HLA-A02:01&B2M&P53 R175H (HMTEVVRHC) Tetramer Protein is expressed from E. coli* with His tag and Avi tag at the C-terminus. APC-Labeled Human HLA-A02:01&B2M&P53 R175H (HMTEVVRHC) Tetramer is assembled by biotinylated monomer and APC-Labeled streptavidin. It contains Gly25-Thr305 (HLA-A02:01), Ile21-Met119 (B2M) and HMTEVVRHC peptide.
Size	100/500 μg
Format	Liquid. Supplied as 0.22 μm filtered solution in 20 mM Tris, 200 mM NaCl, 0.2% BSA (pH 8.0).
Storage	Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Temp	Shipped at ambient temperature.
Lead Time	Product delivery time depends on the supplier.
Species	Human
Expression Host	E. coli
Accession No	A0A140T913(HLA-A*02:01)&P61769(B2M)&HMTEVVRHC
Tag	C-His-Avi
Tag Position	C-terminus
Molecular Type	Tetramer
Gene Synonyms	MHC; HLA-A; P53; TP53; Antigen NY-CO-13; BCC7; FLJ92943; LFS1; TRP53
Amino Acid Range	Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M), and HMTEVVRHC peptide
Activity	Immobilized anti-HLA-A02:01&B2M&P53 R175H antibody, hFc tag at 2 μg/mL (100 μL/well) on the plate. Dose response curve for APC-labeled human HLA-A02:01&B2M&P53 R175H (HMTEVVRHC) tetramer, His tag with the EC50 of 21.1 ng/mL determined by ELISA.
Product Background	p53 is a tumor suppressor protein. Under stressful conditions, p53 tightly regulates cell growth by promoting apoptosis and DNA repair. When p53 becomes mutated, it loses its function, resulting in abnormal cell proliferation and tumor progression. Depending on the p53 mutation, it has been shown to form aggregates leading to negative gain of function of the protein. p53 mutant associated aggregation has been observed in several cancer tissues and has been shown to promote tumor growth.
Allele	HLA-A*02:01
Biotinylated	No

For research or industrial raw materials, not for personal medical use!